Fig. 6

Screen of α1,2-fucosyltransferase to improve 2′-FL production. a An evolutionary tree of ten FucT enzymes. b Effects of replacement of FucT in plasmid P21 with chassis host S04. The recombinant strains RFL24, RFL26–RFL34 were induced and then suspended in a bioconversion mixture containing 50 mM fructose and 50 mM lactose, and the bioconversions were performed for 8 h at 37 ℃ and 220 rpm