Fig. 2

Host strain selection. a Schematic diagram of P01 containing the genes encoding FucT, Gmd and WcaG, and P02 containing the genes encoding ManC and ManB. P_BAD, an araBAD promoter; RBS, ribosome binding sites; Ori, plasmid replication initiation site; Kan^R, kanamycin resistance gene; Str^R, streptomycin resistance gene. b Production of 2′-FL in different chassis strains. The recombinant strains transformed with plasmids P01 and P02 were induced and then suspended in a bioconversion mixture containing 50 mM fructose and 50 mM lactose. The bioconversions were performed for 8 h at 37 ℃ and 220 rpm