From: Development and characterization of a novel nanobody with SRMV neutralizing activity
Primers | Sequence 5’-3’ | Note |
---|---|---|
VHH1-F | GTCCTGGCTGCTCTTCTACAAGG | For library construction, first round of PCR reaction |
VHH1-R | GGTACGTGCTGTTGAACTGTTCC | |
VHH2-F | CTGGCCCAGCCGGCCATGGCTCAGTTGCAGCTCGTG | For library construction, second round of PCR reaction |
VHH2-R | AAGGAAAAAAGCGGCCGCTGAGGAAACGGTGACCTGGG | |
Detc-F | CCATGATTACGCCAAGCTTTGGAGCC | For positive rate identification of the library |
Detc-R | CGATCTAAAGTTTTGTCGTCTTTCC | |
Exp-F | CGCGGATCCATGGCTCAGGTGCAGCTCA | For expression of nanobodies against SRMV |
Exp-R | CCCAAGCTTTGGTTGTGGTTTTGGTGTCTTGGG |