Substrate | CDW (g L−1) | PHB (%) |
---|
Adipate | 1.52 ± 0.05 | 1.16 ± 0.06 |
Pimelate | 0.23 ± 0.01 | 15.14 ± 0.05 |
Suberate | 1.52 ± 0.04 | 1.56 ± 0.19 |
Azelate | 1.47 ± 0.01 | 6.53 ± 0.92 |
Sebacate | 1.52 ± 0.02 | 2.35 ± 0.07 |
1,6-Hexanediol | 1.29 ± 0.14 | 3.59 ± 0.01 |
1,7-Heptanediol | 0.26 ± 0.01 | 21.86 ± 2.77 |
1,8-Octanediol | 0.56 ± 0.01 | 2.20 ± 0.76 |
Mock hydrolysate | 1.16 ± 0.01 | 3.59 ± 0.01 |
- The CDW and PHB content are shown. The strain was cultivated in MSM supplemented with C-equimolar concentrations of pure substrates and 1 mM cyclohexanone as inducer for phaCAB expression. The strain was cultivated in MSM supplemented with C-mol equimolar concentrations to 30 mM of adipate using a C:N ratio of 30:1. The mock hydrolysate consisted of 5 mM of each individual substrate. Error values are calculated as standard deviations (n = 2). Exemplary GC chromatograms are shown in Fig. S7