Fig. 6

Amino acid metabolic functional annotation and intestinal community function network modeling of the altered microbiota. A Cluster analysis of KEGG secondary function. B KEGG secondary function difference analysis. C Amino acid metabolism pathway analysis of KO/KEGG and EC enzymes/MetaCyc. D The network modeling of microbiota and amino acid metabolic function. The microbiota interaction network based on microbiota species abundance and Spearmen’s correlation in all samples (R ≥ 0.7, FDR < 0.05). The core microbiota function network based on microbiota species abundance significance between cold and humid environmental stressed mice and normal control mice (Wilcoxon signed-rank test, FDR < 0.05) and Spearmen’s correlation in all samples (R ≥ 0.7, FDR < 0.05). The microbiota amino acid metabolic function network based on microbiota species abundance, KEGG pathway, MetaCyc pathway, and Spearmen’s correlation in all samples (R ≥ 0.7, FDR < 0.05). Circled, arrowed, and squared shapes are related to microbiota species, KEGG pathway, and MetaCyc pathway, respectively. Red and blue nodes are related to up-regulated and down-regulated abundance significance between cold and humid environmental stressed mice and normal control mice (Wilcoxon signed-rank test, FDR < 0.05), respectively; and gray circled nodes represent microbiota species with no difference. Based on R value of Spearmen correlation, the red and blue edges represent negative and positive correlation, respectively; and the width of the edge represents correlation coefficient; the greater the width, the stronger the correlation