Fig. 4

OTU annotation, gene richness, and microbial compositional changes. A OTU classification VEEN diagram. B The classification of OTU at the level of phylum, class, order, family, genus, and species. C Distribution illustration of samples gene representative of OTU number in community (ace index), community richness (chao1 index), species number in community (observed species index), phylogenetic tree distance in community (PD whole tree index), and community diversity (Shannon index and Simpson index). D Intestinal mucosal microbiota enterotype of cold and humid environmental stressed mice samples. Non-metric multidimensional scaling (NMDS) plot (1), principle component analysis (PCA) plot (2), principal coordinates analysis (PCoA) plot (3), and weighted PCoA plot (4) represent cold and humid environmental stressed mice samples highlighted by intestinal mucosal microbiota enterotype. E Difference analysis of dominant microbiota composition at the level of phylum (1), class (2), order (3), family (4), genus (5), and species (6). CW-Cm, intestinal mucosa samples of the normal control group; CW-Mm, intestinal mucosa samples of cold and humid environmental stress treatment group. CW-Cm vs. CW-Mm, Wilcoxon signed-rank test, *P < 0.05, **P < 0.01