Fig. 2

Intervention of intestinal microbiota disturbance on intestinal mucus barrier in cold and humid environmental stressed mice. A Intervention of intestinal microbiota disorder modeling on culturable bacteria in intestinal of mice with cold and humid environmental stress. On the 6th day of modeling, the bacterial culturing of fecal samples (10 times dilution) of CW-C group, CW-M group, and MD + CW group. up, in 37 ℃ incubator; down, in 37 ℃ anaerobic tank; the dilution was—3. B Effect of intestinal microbiota disorder modeling on the number of culturable bacterial colonies in feces, intestinal contents, and intestinal mucosa. (1) in 37 ℃ incubator; (2) in 37 ℃ anaerobic tank. C Effect of intestinal microbiota disorder modeling on FDA in feces, intestinal contents, and intestinal mucosa. D Effect of intestinal microbiota homeostasis on the intestinal mucus layer. (1) dissect longitudinally colonic tissue and expose the inner layer of mucosa; (2) Hematoxylin–eosin staining of colon tissue, 20 × 10; (3) AB-PAS staining of colon tissue, 20 × 10; the number of goblet cells (4), the thickness of mucus layer (5), and Muc2 (6) in intestinal tissue. CW-C, normal control group; CW-M, cold and humid environmental stress treatment group; MD + CW, microbiota disorder + cold and humid environmental stress treatment group. CW-C vs. CW-M, #P < 0.05, ##P < 0.01. CW-M vs. MD + CW, *P < 0.05, **P < 0.01