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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editing

Fig. 5

Analysis of Poria cocos mutant strains by ultra-high-performance liquid chromatography-quadruple time of flight-dual mass spectrometry (UHPLC-QTOF-MS/MS) analysis. A Total ion chromatography (TIC) of P. cocos mutant strains and triterpene acid standards. WT denotes the wild-type strain, Tr represents the transformed un-knocked strains, Mu1-Mu5 indicate the mutants. 1, 16α-hydroxydehydrotrametenolic acid (HDTRA); 2, poricoic acid B (PAB); 3, dehydrotumulosic acid (DTUA); 4, polyporenic acid C (PAC); 5, poricoic acid AM (PAAM); 6, 3-O-acetyl-16α-hydroxydehydrotrametenolic acid (AHDTRA), 7, 3-O-acetyl-16α-hydroxytrametenolic acid (AHTRA); 8, dehydropachymic acid (DPA); 9, pachymic acid (PA). B The MS/ MS spectrum of dehydropachymic acid (DPA) from HPLC-QTOF-MS/MS

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Microbial Cell Factories

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