How do the carbon and nitrogen sources affect the synthesis of β-(1,3/1,6)-glucan, its structure and the susceptibility of Candida utilis yeast cells to immunolabelling with β-(1,3)-glucan monoclonal antibodies?

Table 7 Glycogen content estimation in the glucan (glucan–glycogen complex) based on ¹H NMR analysis

Sample	H1SC	H1BB	H1gly	Glycogen/%	Glucan/%	Glucan–glycogen ratio
YPG	101	1000	260	19	81	4.2:1
YP_Glyc 10%	198	1000	718	37	63	1.7:1
DPJW_Glyc 7.5%	136	1000	529	32	68	2.1:1
DPJW_Glyc 10%	114	1000	292	21	79	3.8:1
YP_Glyc 10% + Glc 1.5%	203	1000	608	34	66	2.0:1
DPJW_Glyc 10% + Glc 1.5%	140	1000	442	28	72	2.6:1
YP_Malt 10%	63	1000	496	32	68	2.1:1
DPJW_Malt 10%	59	1000	464	30	70	2.3:1

YPG yeast extract + pepton + 2% glucose medium, YP yeast extract + peptone, DPJW deproteinated potato juice water, Glyc glycerol, Glc glucose, Gal galactose, Malt maltose

ISSN: 1475-2859