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Table 6 Individual peaks relative quantity, side-chain length (SC) and branching frequency (BF) parameters obtained from NMR using equations (SC = H6SC / (H6SC – H1SC)) and (BF = H1BB / (H6SC – H1SC) and estimation of relative quantity of β-(1,3)- and β-(1,6)-chains in glucan preparations isolated from chosen biomass after 48 h of cultivation

From: How do the carbon and nitrogen sources affect the synthesis of β-(1,3/1,6)-glucan, its structure and the susceptibility of Candida utilis yeast cells to immunolabelling with β-(1,3)-glucan monoclonal antibodies?

Sample

H6SC

H1SC

H1BB

SC

BF

H1SC/H6SC

β-1,3-/%

β-1,6-/%

YPG

133

101

1000

4.1

31

0.76

88

12

YP_Glyc 10%

226

198

1000

8.1

36

0.88

82

18

DPJW_Glyc 7.5%

177

136

1000

4.4

25

0.77

85

15

DPJW_Glyc 10%

148

114

1000

4.3

29

0.77

87

13

YP_Glyc 10% + Glc 1.5%

264

203

1000

4.4

17

0.77

79

21

DPJW_Glyc 10% + Glc 1.5%

188

140

1000

3.9

21

0.74

84

16

YP_Malt 10%

76.9

62.7

1000

5.4

71

0.82

93

7.1

DPJW_Malt 10%

88.8

58.9

1000

3.0

33

0.66

92

8.2

  1. YPG yeast extract + pepton + 2% glucose medium, YP yeast extract + peptone, DPJW deproteinated potato juice water, Glyc glycerol, Glc glucose, Gal galactose, Malt maltose