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Table 2 The content of total-, α-, β-glucans and trehalose in the dry biomass of studied yeast after cultivation in different growth media

From: How do the carbon and nitrogen sources affect the synthesis of β-(1,3/1,6)-glucan, its structure and the susceptibility of Candida utilis yeast cells to immunolabelling with β-(1,3)-glucan monoclonal antibodies?

Sample

Total glucans [g/100 g dry biomass]

α-glucan [g/100 g biomass]

β-(1,3/1,6)-glucan [g/100 g biomass]

β-(1,3/1,6)-glucan yield

[g/L culture]

Trehalose

[g/100 g biomass]

YPG

24.50 ± 0.94 a,b*

0.98 ± 0.09 a

23.52 ± 1.03 b

2.48 ± 0.55 a

3.77 ± 0.96 e,f

YP_Glc 10%

29.22 ± 1.12 c

5.19 ± 0.06 c

24.03 ± 1.06 b

3.27 ± 0.19 a,b

5.34 ± 1.79 g

DPJW_Glc 10%

29.49 ± 0.16 c

8.96 ± 0.50 e

20.52 ± 0.66 a

4.77 ± 0.17 d,e

5.50 ± 0.75 g

YP_Glyc 10%

23.17 ± 0.42 a

0.95 ± 0.19 a

22.21 ± 0.61 a,b

3.64 ± 0.95 b,c

1.78 ± 0.70 b,c

DPJW_Glyc 7,5%

29.41 ± 0.92 c

1.05 ± 0.28 a

28.36 ± 1.20 d

6.98 ± 0.54 g

1.90 ± 0.33 b,c

DPJW_Glyc 10%

28.32 ± 1.34 c

1.23 ± 0.01 a,b

27.10 ± 1.33 c,d

7.03 ± 0.95 g

1.34 ± 0.56 b

YP_Glyc 10% + Glc 1.5%

23.98 ± 2.34 a,b

0.92 ± 0.02 a

23.21 ± 2.64 b

4.60 ± 0.21 c,d,e

2.97 ± 0.31 d

DPJW_Glyc 10% + Glc 1.5%

25.17 ± 0.66 a,b

1.22 ± 0.01 a,b

23.94 ± 0.65 b

5.62 ± 1.08 e,f

1.58 ± 0.52 b

YP_Gal 10%

26.06 ± 1.60 b

1.32 ± 0.02 a,b

24.74 ± 1.58 b,c

2.57 ± 0.60 a

3.03 ± 0.90 d,e

DPJW_Gal 10%

25.94 ± 0.20 b

1.76 ± 0.03 b

24.18 ± 0.16 b,c

4.21 ± 0.49 b,c,d

0.45 ± 0.19 a

YP_Malt 10%

29.75 ± 1.47 c

7.27 ± 0.56 d

22.49 ± 0.91 a,b

3.91 ± 0.65 b,c,d

4.48 ± 1.04 f

DPJW_Malt 10%

32.72 ± 0.69 d

10.20 ± 0.31 f

22.51 ± 0.38 a,b

6.43 ± 1.15 f,g

2.48 ± 0.32 c,d

  1. YPG yeast extract + pepton + 2% glucose medium, YP yeast extract + peptone, DPJW deproteinated potato juice water, Glyc glycerol, Glc glucose, Gal galactose, Malt – maltose; * a, b, c, … mean values in column marked with the same letters do not differ significantly