Fig. 5

Secreted A5 4mer silk titer for strain Yngk in LB, M9, and supplemented M9 media. Expressions were carried out for 21 h at 37 °C with 1.5% w/v xylose supplemented for recombinant gene induction. All M9 media experiments were performed with 1% w/v glucose as a carbon and energy source. Expressions were performed in supplemented M9 minimal media that contained 0.3 g/L of glutamate (+ 1 aa), 0.3 g/L of histidine, isoleucine, phenylalanine, proline, tyrosine, lysine, and methionine (+ 7 aa), 0.3 g/L of glutamate, histidine, isoleucine, phenylalanine, proline, tyrosine, lysine, and methionine (+ 8 aa), 10 mM sodium acetate at time of induction (+ 10 mM), 30 mM sodium acetate at time of induction (+ 30 mM), and 90 mM sodium acetate at time of induction (+ 90 mM). Error bars represent standard deviations from the mean values of three replicates