Fig. 3

A5 4mer secreted titer for strains with altered TISs compared to strains Yngk and Yoch in LB and M9 media. The M9 minimal media was supplemented with 1% w/v glucose as a carbon and energy source. Strains with an asterisk (*) were unable to grow overnight in M9 media when cultures were inoculated from a single colony, these strains were also observed to have an extended lag phase when expression cultures in M9 minimal media were inoculated from overnight cultures grown in LB media. All data was generated from 21-h expressions at 37 °C with 1.5% w/v xylose supplemented for recombinant gene induction. Error bars represent standard deviations from the mean values of three replicates