Fig. 1

SDS PAGE from nickel-chromatography purification on supernatants of strain Yngk and Yoch. Lanes: (L) Protein ladder with kDa values listed to the left. (Yngk SS) Flow through of Yngk (FT), Wash 1 of Yngk (W1), Wash 2 of Yngk (W2), Elution of Yngk (E). (Yoch SS) Flow through of Yoch (FT), Wash 1 of Yoch (W1), Wash 2 of Yoch (W2), Elution of Yoch (E). The purified A5 4mer silk can be found in the elution lanes for both SSs (at approximately 60 kDa). Inefficient Coomassie staining of the secreted A5 4mer protein was observed