Table 1 Comparison of selected whole-cell based production systems of 25OHVitD₃

Escherichia coli BL21 (DE3)

recombinant CYP105A1

100

dimethylsulfoxid, 1% (v/v)

2.5

24 h

[21]

Kutzneria albida

CYP

500

HPCD, 1% (w/v)

70.4

48 h

[22]

Bacillus megaterium

recombinant CYP109A2 (T103A mutant)

577

HPCD, 2.25% (w/v)

saponin, 0.0825% (w/v)

282.7

48 h

[23]

Pseudonocardia sp. KCTC 1029BP

n.d.

600

ethanol, 0.2% (v/v)

HPCD, 0.1% (w/v)

356

120 h

[24]

Rhodococcus erythropolis

recombinant CYP (T107A mutant)

769

partially methylated β-cyclodextrin, 1.5% (w/v)

573

2 h

[18]

Bacillus cereus zju 4-2

n.d.

2000

propylene glycol, 9% (v/v)

ethanol, 1% (v/v)

830

60 h

[17]

Thauera aromatica K172

recombinant S25DH₁

1780

HPCD, 12.5% (w/v)

ethanol, 5% (v/v)

1850

50 h

this work