Fig. 6
HPCD recovery. (A), General recycling setup. (B), Experimentally determined HPCD concentrations before and after ten consecutive VitD3 conversions. The black symbols represent the maximum expected HPCD concentration taking into account a 7% loss due to sampling and removal of cells and product. (C), Relative product formation in samples with freshly added HPCD (5% [w/v]) vs. recycled HPCD in ten consecutive VitD3 conversions within 4 h (0.5 mL scale, OD 100 corresponding to 16 mg cells [dry weight], 1% [v/v] isopropanol, aerobic). 100% refer to the respective formation of 25OHVitD3 with freshly added HPCD in each cycle