Fig. 5From: Highly selective whole-cell 25-hydroxyvitamin D3 synthesis using molybdenum-dependent C25-steroid dehydrogenase and cyclodextrin recyclingTime course of optimised conversion of 4.63 mM VitD3 to 25OHVitD3 using S25DH1-producing resting T. aromatica cells (100 mL scale, OD 100 corresponding to 3.2 g cells [dry weight], 12.5% [w/v] HPCD, 5% [v/v] ethanol, 50 mM K3[Fe(CN)6]). Electron-accepting K3[Fe(CN)6] was added in 10 mM increments (green shading)Back to article page