Fig. 4
Effect of HPCD and isopropanol on whole-cell conversion and solubilisation of VitD3. (A), Effect of different HPCD concentrations on the formation of 25OHVitD3 from 500 µM VitD3 (0.25 mL scale, OD 50 corresponding to 4 mg cells [dry weight], 1% [v/v] isopropanol, aerobic). (B), Experimentally determined VitD3 concentrations in aqueous buffer using ethanol as co-solvent and HPCD