Fig. 2
Electron acceptor-dependent whole-cell conversion of 500 µM VitD3 to 25OHVitD3 by recombinant T. aromatica cells producing S25DH1. (A), Electron acceptor dependence (0.25 mL scale, OD 50 corresponding to 4 mg cells [dry weight], 5% [w/v] HPCD, 1% [v/v] isopropanol). (B), S25DH1 activity in cell free extracts and whole cells (0.1 mL scale, 0.1–0.3 mg total protein [whole cell or cell extract] 10% [w/v] HPCD, 1% [w/v] isopropanol, 10 mM K3[Fe(CN)6]); 1 mU refers to 1 nmol min–1. (C), Malate dehydrogenase (MDH) activity in cell free extracts and whole cells; 1 mU refers to 1 nmol min–1. (D), Fluorescence microscopy visualizing the ratio of living (green) and dead (red) cells