Fig. 6
(A) Left: 5-ml MRS cultures based on Ptec_rep and Ptec bacteria. Cells were pelleted down, and pellets are shown at the bottom. Right: 95-ml LB (supplemented with 5 ml 1 M glucose) cultures based on Ptec_rep and Ptec bacteria. Cells were pelleted down, and the corresponding pellets are shown at the bottom. (B) Pellets of bacteria encoded to express mCherry driven by Ptec_rep, Ptec and PtlpA after overnight growth. (C) Fluorescence microscopy images of bacteria encoded to express mCherry driven by Ptec_rep, Ptec and PtlpA. (D) FACS data showing the comparison between bacteria encoded to express mCherry driven by Ptec _rep, Ptec and PtlpA. (E) Expression levels of mCherry driven by Ptec in L. plantarum WCSF1, PtlpA in L. plantarum WCSF1 and PtlpA in E. coli Nissle 1917. Fold changes of mCherry expression driven by Ptec in L. plantarum WCSF1 and PtlpA in E. coli Nissle normalized to the expression in PtlpAL. plantarum WCSF1. All the experiments were performed as experimental triplicates (N = 3). Column heights and error bars represent the means and SD. ** p < 0.01, *** p < 0.001