Fig. 5
(A) Scheme of the genetic modifications that introduced the operator of rep at different locations in and around the promoter PtlpA. (B) Expression levels of mCherry for all three operator variants (with and without the rep repressor in the plasmid). (C) Percentage of repression mediated by each operator variant (O1, O2 and O3). (D) Scheme of the substitution of PtlpA by Ptec. (E) Expression levels of mCherry for Ptec_mCherry and Ptec_rep_mCherry bacteria. (F) Percentage of repression and fold-changes for PtlpA_O_rep (OR_rep), PtlpA_O3_rep (O3_rep) and Ptec _rep. All the experiments were performed as experimental triplicates (N = 3). Column heights and error bars represent the means and SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001