Fig. 4
(A) Scheme of the site-directed mutagenesis done to remove the operator from the plasmid. (B) Expression levels of mCherry in terms of RFU for PtlpA (unmodified), PtlpA_rep (only repressor), PtlpA_op (only rep operator) and PtlpA_op_rep (operator plus rep). All the experiments were performed as experimental triplicates (N = 3). Column heights and error bars represent the means and SD. ns = not significant, ***p < 0.001. (C) AlphaFold 3D structure prediction of the rep as a monomer. (D) AlphaFold 3D structure prediction of the rep as a dimer