Fig. 3
(A) Expression levels of mCherry in relative fluorescence units (RFU) for all three operators (O cng, O rep and O cI) and all three operators plus repressors (cng, rep and cI). (B) Percentage of repression mediated by each repressor. (C) Growth curves of all three repressors and wild-type bacteria over 16 h. (D) Bacterial biomass of wild-type, PtlpA_mCherry, and bacteria carrying each repressor after overnight growth in the incubator. Each sample is based on a 5-mL culture. Experiments for Figures A, B and D were performed as experimental triplicates (N = 3). Experiments for Figure C were performed as experimental duplicates, each with two technical replicates (N = 2, n = 2). Column heights and error bars represent the means and standard deviations (SD). ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001