Fig. 2
(A) Cloning workflow design. In the first round of cloning, the operators were inserted within the promoter PtlpA. In the second round, the repressors were cloned in the corresponding plasmid. All genetic fragments were based on IDT synthetic eBlocks. (B) Flow cytometry data showing the effect on mCherry expression of cloning each operator (O) and each operator plus repressor (O + R) in the plasmid