Fig. 4

Improved recombinant FAST production with strain ‘B2’ resulting in a homogeneous fluorescent cell population. A Fluorescent intensity of the whole cell population of recombinant ‘B2’ strains determined using a microplate reader in presence or absence of the fluorogen ^TFLime. Lactose-induced feg expression controlled by P_bgaL caused weak and constitutive expression controlled by P_fd strong fluorescence. No fluorescence was determined in absence of.^TFLime, by the empty vector control, or by non-induced cells. B Number of fluorescent recombinant ‘B2’ cells determined using flow cytometry. C Density plots of recombinant ‘B2’ strains. All ‘B2’cells harboring the empty vector control pJIR751 were non-fluorescent. Lactose-induced expression of feg caused an overall weak fluorescent heterogeneous population. Expression of feg controlled by the constitutive P_fd promoter resulted in a homogeneous, brightly fluorescent population of recombinant ‘B2’ [pJIR751_Pfd_FAST] cells. Error bars indicate standard deviations. n = 3