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Table 1 Substrate specificity of chitinases from M. pulcherrima

From: Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima

  

Relative Activity (%)

Chitinous Material

MW (kDa); DD (%)

MpChit35

MpChit38

MpChit41

Colloidal chitin

n.d; ≤ 5

100.0 ± 10.0

100.0 ± 11.2

100.0 ± 8.0

Chitosan CHIT50.1

75–125; ≥ 92

16.0 ± 2.0

14.0 ± 3.1

20.0 ± 5.3

Chitosan CHIT50.2

50–190; ≥ 75

72.2 ± 6.3

50.0 ± 2.4

72.0 ± 4.1

Chitosan CHIT100

100–300; ≥ 92

5.1 ± 1.2

n.ad.

n.ad.

Chitosan CHIT600

100–300; ≥ 92

10.0 ± 3.4

n.ad.

n.ad.

  1. Values are the percentages of the maximum activity assayed for each enzyme type; activity data are average of 3 assays and standard errors are indicated; 100% activity: 0.4 U ml− 1, 0.5 U ml− 1 and 0.7 U ml− 1 for MpChit35, MpChit38 and MpChit41, respectively; DD, deacetylation degree; n.d., not determined; n.ad., no activity detected