Fig. 3
From: Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima
Analysis of the chitinases glycosylation on SDS-PAGE. (a) MpChit35 (lines 1 and 2), MpChit38 (lines 3 and 4) and MpChit41 (lines 5 and 6) before (-) and after (+) 24 h digestion with PNGase F (black arrow, 36 kDa). Samples were prepared from concentrated 144 h culture filtrates. Numbers on the left indicate the positions of molecular mass standards (lane M) in kDa. (b) Representative peptides identified by RP-LC-MS/MS of the indicated chitinases in the protein bans of about 36 kD (line 2) and 38 kDa (line 4) of panel (a)