Fig. 1

Analysis of chitinase activity on plate and heterologous expression of the M. pulcherrima chitinases. (a) Chitinolytic activity assay on solid BPC medium of P. pastoris (1) as negative control, M. pulcherrima (2), and transformants of P. pastoris expressing chitinases MpChit35 (3), MpChit38 (4) and MpChit41 (5). (b) Activity profiles of P. pastoris cultures expressing the referred chitinases from M. pulcherrima after methanol induction during the indicated times. Cell growth (OD600, dashed lines), pH (diamonds) and extracellular chitinase activity (solid lines) of the transformants expressing the referred proteins using colloidal chitin as substrate. Activity of P. pastoris culture including pIB4 vector was used as negative control (empty circles). Each point of activity represents the average of three independent measurements and standards errors are indicated