Fig. 5

Double deletion mutants with increased laccase activity. (A) Laccase activity of the indicated strains from a double deletion mini-array were assessed on three 96-well plates using the ABTS liquid culture assay after 4 days of growth. Three biological replicates were assessed for each double mutant with additional controls. Normalized laccase activity was averaged and compared to the activity levels from the reference strain. Gene deletions were either done by integrating the hygromycin (hph; blue) or kanamycin (kan; red) resistance modules. (B) Laccase activity of the indicated strains grown on the same 96 well plate with three biological replicates. ONE way ANOVA tests with Tukey’s correction were performed and adjusted p values are shown for comparisons between double deletion mutants and corresponding single deletion mutant strains integrated with either the hph (blue) or kan (red) modules (adjusted p values; < 0.05: *; <0.01: **: <0.005: ***)