Fig. 3

Change of laccase activity confirmed in liquid culture. A. Positive hits identified in the first overlay screen were assessed in liquid cultures. The strains were grown and assessed on three separate sets (plates 1–3) and ranked based on the average laccase activity (µmol/min) normalized to cell density. For each strain, three biological replicates were grown on the same plate and laccase activity was compared to the respective parental query strain (i.e., grown on the same plate) using ONE way ANOVA tests with Dunnett’s corrections (adjusted p values; < 0.05: *; <0.01: **: <0.005: ***). Filled circles represent data significantly different in comparison to reference samples. B and C. A subset of negative hits were assessed in liquid cultures. Three biological replicates for each strain were assessed and ranked based on the average laccase activity normalized to cell density as in A. Filled circles represent results significantly different from reference samples