Fig. 1

Screening of the YKO collection for laccase expression. (A) Schematic of the establishment of a library with single gene deletion mutants expressing laccase using the SGA methodology. Approximately 5,000 strains were mated with the JHY716_ttLCC1 query strain producing a total of 4,790 unique laccase-expressing single gene deletion mutant strains. (B) Workflow of the ABTS overlay assay. (C) Images of a representative array (plate 11, replicate 3) before washing (left) and an hour after addition of the overlay (right). Sites with differing amount of laccase activity can be identified by the intensities of the colorimetric signal