Fig. 5

Western blot analysis of cell-free extracts from recombinant KW1 (A) and KW2 (B) strains producing H56 fused to various anchoring sequences. About 0.8 µg of protein was loaded for the KW1 strains, while 1.6 µg was loaded of most KW2 strains, except the lanes labeled NTTM-H56 and LysM-H56 which contain 2.4 µg of protein. Well 1: Magic Marker, well 2: pEV, well 3: Cyt-H56 (predicted protein size ~ 50 kDa, both strains), well 4: NTTM-H56 (KW1: 60 kDa, KW2: ~65 kDa), well 5: Lipo-H56 (~ 58 kDa, both strains), well 6: LysM-H56 (KW1: 60 kDa, KW2: ~86 kDa), well 7: LPxTG-H56 (~ 78 kDa, both strains). The arrows indicate the fusion protein