Fig. 4

Growth curves for recombinant KW1 (A) and KW2 (B) strains. Induced cultures of L. pentosus KW1 (A) or KW2 (B) carrying various expression plasmids were transferred to a sterile 96-well plate directly after induction (time point 0 h), and the OD₆₀₀ of the cultures was continuously measured every 15 min for 24 h at 37 °C. The data shown are a mean of three biological replicates; standard deviations are indicated by shading. In the absence of induction, all strains showed the same growth curves, similar to those of induced pEV (data not shown)