Fig. 1

Phenotyping on pure and crude glycerol at 1 mL scale. Strain U. maydis MB215 ∆cyp3 ∆MEL ∆UA ∆dgat ∆P_ria1::P_etef ∆fuz7 P_etefmttA was grown in defined MTM with 125 g L^-1 crude or pure glycerol as the sole carbon source and 2.4 g L^-1 NH₄Cl. The cultivation was performed in a BioLector Pro under the following conditions: 1400 rpm, 30 °C, 85% humidity, online measurements were taken every 10 min, sacrifice samples were taken in irregular intervals during the cultivation. Metabolites were analysed in duplicate via HPLC