Table 1 Strains and plasmids used in this study
Strains/plasmids | Description/genotype | References |
|---|---|---|
Strains | ||
Escherichia coli | ||
S17-1 | thi pro hsdR recA chromosomal RP4; Tra+; Tmpr Str/Spcr | Simon et al. [33] |
Ralstonia eutropha | ||
H16 | Wild type | DSM 428 |
NSDG-GG | H16 derivative; ∆phaC::phaCNSDG, ΔnagR, nagE(G793C), PA2858 -glpFKEc-h16_A2858 | Zhang et al. [15] |
ΔB1 | NSDG-GG derivative; ΔphaB1Re | Mifune et al. [34] |
ΔB1ΔB3 | NSDG-GGΔB1 derivative; ΔphaB3 | This study |
ΔB1ΔB3ΔB2-C2 | NSDG-GGΔB1ΔB3 derivative; ΔphaB2-phaC2 | This study |
ΔB1Δhad | NSDG-GGΔB1 derivative; Δhad | This study |
ΔB1ΔpaaH1 | NSDG-GGΔB1 derivative; ΔpaaH1 | This study |
ΔB1Δcrt2 | NSDG-GGΔB1 derivative; Δcrt2 | This study |
ΔB1ΔhadΔpaaH1 | NSDG-GGΔB1Δhad derivative; ΔpaaH1 | This study |
ΔB1ΔhadΔpaaH1Δcrt2 | NSDG-GGΔB1ΔhadΔpaaH1 derivative; Δcrt2 | This study |
∆B1∆A3330 | NSDG-GGΔB1 derivative; Δh16_A3330 | This study |
ΔB1ΔbktB | NSDG-GGΔB1 derivative; ΔbktB | This study |
ΔB1ΔphaJ4a | NSDG-GGΔB1 derivative; ΔphaJ4a | This study |
∆B1∆fadB’ | NSDG-GGΔB1 derivative; ΔfadB’ | This study |
Plasmids | ||
pK18mobsacB | pMB1 ori, mob, Kanr, sacB | Schafer et al. [35] |
pk18msΔB3 | pK18mobsacB derivative; phaB3 del | Insomphun et al. [14] |
pk18msΔB2-C2 | pK18mobsacB derivative; phaB2-phaC2 del | Subagyo et al. [37] |
pk18msΔhad | pK18mobsacB derivative; had del | Segawa et al. [20] |
pk18msΔpaaH1 | pK18mobsacB derivative; paaH1 del | Segawa et al. [20] |
pk18msΔcrt2 | pK18mobsacB derivative; crt2 del | Segawa et al. [20] |
pk18msΔA3330 | pK18mobsacB derivative; h16_A3330 del | This study |
pk18msΔbktB | pK18mobsacB derivative; bktB del | This study |
pk18msΔphaJ4a | pK18mobsacB derivative; phaJ4a del | Kawashima et al. [25] |
pK18ms∆fadB’ | pK18mobsacB derivative; fadB’ del | Insomphun et al. [12] |
pBPP-ccrMe-phaJ4a-emd | pBBR ori, mob, PphaP1, ccrMe, phaJ4a, emdMm, TrrnB | Insomphun et al. [14] |
pBPP-ccrMe-phaJAc-emd | pBBR ori, mob, PphaP1, ccrMe, phaJAc, emdMm, TrrnB | Insomphun et al. [14] |