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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Microaerobic insights into production of polyhydroxyalkanoates containing 3-hydroxyhexanoate via native reverse β-oxidation from glucose in Ralstonia eutropha H16

Fig. 5

Metabolic pathways for P(3HB-co-3HHx) biosynthesis in R. eutropha under the low-aerated conditions. Promoted PHA biosynthesis in R. eutropha H16 under slow-shaking condition could be due to the presence of PhaB-independent pathway such as by enigmatic NAD(P)H-dependent (R)-specific reductase, or (S)-specific route mediated by other isologs of (S)-3HB-CoA dehydrogenase(s) and crotonase(s). The microaerobic cultivation has conditionally activated the native rBOX pathway as well, leading to the formation of (R)-3HHx-CoA. Further introduction of heterologous Ccr-PhaJ-Emd (artificial rBOX) showed concerted effect with native rBOX on P(3HB-co-3HHx) production under aforementioned condition. Black arrows indicate the native pathways whereas purple arrows indicate the plasmid borne artificial pathway

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Microbial Cell Factories

ISSN: 1475-2859

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