Method for plasmid-based antibiotic-free fermentation

Table 1 Plasmids developed and used in this study

Name	Features	Description
CGB285	colE1-infA-chromoprotein-Cm	High copy, infA selection and Cm selection, chromoprotein expression
CGB288	p15A-infA-chromoprotein	Low copy, infA selection, chromoprotein expression
CGB289	colE1-infA-chromoprotein	High copy, infA selection, chromoprotein expression
CGB290	R6K-infA-chromoprotein	Medium copy, infA selection, chromoprotein expression
CGB237	pQE80L-TNFα-infA -Cm	High copy, infA selection and Cm selection, TNFα expression from the T7 promoter

ISSN: 1475-2859