Fig. 1

Schematic representation of the genome modifications to yield arabinose-inducible infA expression. a In the first step, the arabinose repressor, an arabinose-inducible promoter and an FRT-flanked constitutively expressed selection marker cassette were integrated upstream of the infA coding sequence to replace the endogenous infA promoter. b Flp recombinase-mediated excision of the selection marker and its constitutive promoter leads to arabinose-dependent expression of infA. araC, arabinose repressor; P-araC, araC promoter; P-araB, ribulokinase promoter (arabinose-inducible); FRT, flippase recognition target; P-rpsL, promoter from 30 S ribosomal subunit protein S12 (constitutive); Kan^R, kanamycin resistance gene (aminoglycoside phosphotransferase); infA, Translation Initiation Factor 1