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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9

Fig. 1

Homology modelling of the recombinant OmpF-MPER protein. Upper (A) and lateral (B) views of the OmpF protein trimer from E. coli K-12 strain (based on 6wtz.pdb). Upper (C) and lateral (D) views of the predicted OmpF-MPER protein from EcN-MPER, homology modelling is made on 6wtz.pdb structure using SWISS-MODEL tool. Green indicates location of the MPER sequence. (E) Sequence alignment of the OmpF-MPER (OmpF-M) chain A of the EcN-MPER to the OmpF K-12 (6wtz) template. MPER sequence is underlined in bold, arrows indicate transmembrane regions of the porin

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