Table 1 Summary of native promoter functions and rationale for inclusion in the study
From: Saccharomyces boulardii promoters for control of gene expression in vivo
Promoter | Native gene function | Reasoning for selection | % identity to S288C |
|---|---|---|---|
PALD6 | Cytosolic aldehyde dehydrogenase; conversion of acetaldehyde to acetate | Involved in growth on non-fermentable carbon sources. Evidence for stronger expression in S. boulardii than S. cerevisiae | 99.68 |
PCYC1 | Cytochrome c isoform 1. Electron carrier during cellular respiration | Common medium expression promoter | 98.75 |
PCYC7 | Cytochrome c isoform 2. Electron carrier during cellular respiration | Similar function to CYC1, but induced in hypoxic conditions | 97.86 |
PDAN1 | Cell wall mannoprotein | No expression in presence of oxygen | 99.36 |
PHSP26 | Heat shock protein. Chaperone that prevents aggregation of unfolded proteins | High expression in response to heat shock/carbon starvation/stress | 97.50 |
PHXT7 | High-affinity glucose transporter | Low/no expression in presence of glucose. High expression in absence of glucose. Note: low oxygen can lead to reduced expression | 99.90 |
PJEN1 | High-affinity uptake of monocarboxylate carbon sources (e.g. lactate, acetate) | Repressed at high glucose. Expressed in presence of alternative carbon sources e.g. lactate, glycerol and acetate | 97.43 |
PSSA1 | Multifunctional ATPase involved in many aspects of protein regulation | Constitutive promoter. Low expression during exponential growth phase; high expression in post-diauxic phase | 99.33 |
PSUC2 | Invertase, has secreted and intracellular forms. Hydrolyses sucrose to glucose and fructose | High expression in presence of sucrose | 98.90 |
PTDH3 | Glyceraldehyde-3-phosphate dehydrogenase, involved in glycolysis and gluconeogenesis | Strong ‘constitutive’ promoter | 98.97 |
PTEF1 | Translational elongation factor EF-1 alpha; delivers charged tRNAs to the ribosome | Strong ‘constitutive’ promoter | 99.52 |
PTPI1 | Triose phosphate isomerase, involved in glycolysis | Common promoter for protein production. Medium strength | 99.86 |