Fig. 1

Promoter function and characterisation in glucose. A Schematic overview of native roles of promoter genes. B Aerobic growth of the promoter strains over time. C Aerobic relative fluorescence intensity (RFI) of yEGFP produced by the promoter strains over time. Dashed lines in (B) and (C) indicate 8 and 24 h, the time points that were selected for further investigation by flow cytometry. D Mean of the normalised median relative fluorescence intensity of yEGFP from the 8 h time point and (E) from the 24 h time point of the aerobic (blue) and anaerobic (green) flow cytometry experiments. All plots display the mean of three biological replicates, from independent pre-cultures. Bar plots are displayed as the mean ± SD. Points represent individual replicates. * p < 0.05 and ** p < 0.005 (D) and (E) were analysed with t-tests and p-values were adjusted for false discovery rate