Table 2 E. coli strains and plasmids used in this study

strain

MG1655

F−, λ−, rph-1

KCTC

MGI

MG1655 ilvGΔ::PLac-dxs, glvCΔ::PLac-idi, yjbIΔ::PLac-ispA, agaAVΔ::PLac-dxr

[39]

MGI2E

MGI (pfkAΔ::PLac-CrtE, ushAΔ::PLac-CrtE)

This study

ZEA-1

MGI2E (atpIΔ::Ptrc-CrtYIB, ldhAΔ::Ptrc-YZ)

This study

Z1pC

ZEA-1 harboring pSTVM_CsCCD2

This study

Z1pCpA6803

ZEA-1 harboring pSTVM_CsCCD2 and pBBR-A6803

This study

Z1pCpA7942

ZEA-1 harboring pSTVM_CsCCD2 and pBBR-A7942

This study

Z1pCA7942(M)

ZEA-1 harboring pSTVM_CsCCD2_Syn-aldH7942(M)

This study

Z1pCA7942(P)

ZEA-1 harboring pSTVM_CsCCD2_Syn-aldH7942(P)

This study

Z1pCA7942(P)pN

ZEA-1 harboring pSTVM_CsCCD2_Syn-aldH7942(P) and pUCrop_NtUGT

This study

Z1pCA7942(P)pNC

ZEA-1 harboring pSTVM_CsCCD2_Syn-aldH7942(P) and pUCrop_NtUGT_CaUGT3

This study

TOP10

F-mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1

Invitrogen

BL21 (DE3)

F^–ompT gal dcm lon hsdSB(r_B^–m_B^–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB⁺]_K−12(λ^S)

NEB

Synechocystis sp. PCC 6803

wild type strain of Synechocystis sp.PCC6803

Synechocystis elongatus PCC 7942

wild type strain of S. elongatus PCC7942

Plasmid

pKK223-3

Amp^r, tac promoter, pBR322 ori, rop

Pharmacia

pTrc99A

Amp^r, trc promoter, pBR322 ori

[32]

pUCM

Amp^r, constitutively expressed lac promoter, pUC ori

[29]

pUCrop

Amp^r, constitutively expressed lac promoter, pUC ori, rop

[39]

pET21a(+)

Amp^r, T7 promoter, C-terminal His-tag sequence, f1 ori, rop,

Novagen

pSTVM

Cm^r, Cloning/expression vector removing lac promoter from pSTV29

[33]

pBBR1MCS-2

Km^r, Cloning vector

[40]

pUCM_CrtE

Amp^r, pUCM derivative, expression vector for crtE from P. ananatis

[26]

pTrc99A_YIB

Amp^r, pTrc99A derivative, expression vector for crtYIB from P. ananatis crtYIB operon at EcoRI/HindIII site

This study

pKK_CsCCD2

Amp^r, pKK-223-3 derivative, expression vector for CsCCD2 from C.sativus at EcoRI/HindIII site

This study

pSTVM_CsCCD2

Cm^r, pSTVM derivative, expression vector for CsCCD2 from C.sativus under tac promoter at BglII/NotI site

This study

pUCM_Syn-aldH6803

Amp^r, pUCM derivative, expression vector for aldH from Synechocystis sp. PCC6803 (Syn_aldH6803) at XbaI/EcoRI site

This study

pUCM_Syn-aldH7942

Amp^r, pUCM derivative, expression vector for aldH from Synechocystis sp. PCC7942 (Syn_aldH7942) at XbaI/EcoRI site

This study

pBBR-A6803

Km^r, pBBR1MCS-2 derivative, expression vector for aldH from Synechocystis sp. PCC6803 (Syn_aldH6803) under lac promoter

This study

pBBR-A7942

Km^r, pBBR1MCS-2 derivative, expression vector for aldH from S. elongatus PCC7942 (Syn_aldH7942) under lac promoter

This study

pSTVM_CsCCD2_Syn-aldH6803(M)

Cm^r, pSTVM derivative, individual modular expression vector for CsCCD2 and Syn_aldH6803

This study

pSTVM_CsCCD2_Syn-aldH7942(M)

Cm^r, pSTVM derivative, individual modular expression vector for CsCCD2 and Syn_aldH7942

This study

pSTVM_CsCCD2_Syn-aldH6803(P)

Cm^r, pSTVM derivative, polycistronic expression vector for CsCCD2 and Syn_aldH6803

This study

pSTVM_CsCCD2_Syn-aldH7942(P)

Cm^r, pSTVM derivative, polycistronic expression vector for CsCCD2 and Syn_aldH7942

This study

pUCrop_NtUGT

Amp^r, pUCrop derivative, expression vector for NtUGT at XbaI/XmaI site

This study

pUCrop_NtUGT_CaUGT3

Amp^r, pUCrop derivative, expression vector for NtUGT and CaUGT3 at XbaI/NotI site

This study

pET21a(+)_GjUGT1

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged GjUGT gene at EcoRI/XhoI site

This study

pET21a(+)_GT1-316

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged GT1-316 gene at BamHI/SalI site

This study

pET21a(+)_NtUGT

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged NtUGT gene at BamHI/HindIII site

This study

pET21a(+)_FaGT2

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged FaGT2 gene at BamHI/HindIII site

This study

pET21a(+)_StUGT

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged StUGT gene at EcoRI/XhoI site

This study

pET21a(+)_CaUGT3

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged CaUGT3 gene at BamHI/SalI site

This study

pET21a(+)_NsUGT

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged NsUGT gene at BamHI/SalI site

This study

pET21a(+)_SpUGT

Amp^r, pET21a(+) derivative, inducible expression of 6xHis-tagged SpUGT gene at BamHI/SalI site

This study

pMP11

Amp^r, pKD46 with constitutively expressed Cas9, aTc gRNA targeting ColE1 ori

[41]

pgRNA

Cm^r, constitutively expressed sgRNA targeting

[41]

pgRNA_pfkA

Cm^r, constitutively expressed sgRNA targeting pfkA, ColE1 ori

This study

pgRNA_ushA

Cm^r, constitutively expressed sgRNA targeting ushA, ColE1 ori

This study

pgRNA_atpI

Cm^r, constitutively expressed sgRNA targeting atpI, ColE1 ori

This study

pgRNA_ldhA

Cm^r, constitutively expressed sgRNA targeting ldhA, ColE1 ori

This study