Table 1 Plackett-Burman design for Aspergillus awamori MK788209 cellulase production
Trial | Factor 1 A:MS weight g/flask | Factor 2 B:inc. time Day | Factor 3 C: glucose g/l | Factor 4 D: lactose g/l | Factor 5 E: baker’s yeast g/l | Factor 6 F: peptone g/l | Factor 7 G: (NH4)2SO4 g/l | Factor 8 H: CuSO4 g/l | Factor 9 J: FeSO4 g/l | Factor 10 K: CaCl2 g/l | Factor 11 L: KCl g/l | Cellulase activity (U/ml) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 4 | 10 | 0 | 1 | 1 | 0 | 0.01 | 0.01 | 0.01 | 0 | 0.929 |
2 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.864 |
3 | 2 | 4 | 0 | 10 | 0 | 1 | 1 | 0 | 0.01 | 0.01 | 0.01 | 2.053 |
4 | 4 | 7 | 0 | 10 | 1 | 1 | 0 | 0 | 0 | 0.01 | 0 | 3.727 |
5 | 4 | 7 | 0 | 0 | 0 | 1 | 0 | 0.01 | 0.01 | 0 | 0.01 | 4.013 |
6 | 2 | 7 | 0 | 10 | 1 | 0 | 1 | 0.01 | 0.01 | 0 | 0 | 2.522 |
7 | 4 | 4 | 10 | 10 | 1 | 0 | 0 | 0 | 0.01 | 0 | 0.01 | 2.843 |
8 | 4 | 4 | 0 | 0 | 1 | 0 | 1 | 0.01 | 0 | 0.01 | 0.01 | 3.507 |
9 | 4 | 4 | 10 | 10 | 0 | 1 | 1 | 0.01 | 0 | 0 | 0 | 2.613 |
10 | 2 | 7 | 10 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0.01 | 2.369 |
11 | 2 | 7 | 10 | 10 | 0 | 0 | 0 | 0.01 | 0 | 0.01 | 0.01 | 2.969 |
12 | 4 | 7 | 10 | 0 | 0 | 0 | 1 | 0 | 0.01 | 0.01 | 0 | 4.636 |