Table 1 Summary of experimental setup designed with DoE (DX, StatSoft)
Design type: face-centered Central composite design | Runs: 104 for each of 125 TF = 13 000 in duplicate = 26,000 readouts on FL (fluorescence) and growth (OD600) | |||||||||
The central variants were repeated five times. The order of culture execution was randomized | ||||||||||
Process factors | Categoric: A, B, C Numeric: d, e | |||||||||
Factor | Name | Units | Change | Type | Minimum | Maximum | Coded Low | Coded High | Mean | Std. Dev |
|---|---|---|---|---|---|---|---|---|---|---|
A | AIR “limiting” | 0/1 | Easy | Categoric | Low | High | Levels: 2 | |||
B | Carbon source | Glu/Gly | Easy | Categoric | Glucose | Glycerol | Levels: 2 | |||
C | Nitrogen source | i/o | Easy | Categoric | Inorganic: Ammonium Sulfate | Organic: Casamino Acid Hydrolysate | Levels: 2 | |||
d | pH | pH | Hard | Numeric | 3 | 7 | − 1 → 3 | + 1 → 7 | 5 | 2.83 |
e | Temperature | °C | Hard | Numeric | 22 | 34 | − 1 → 22.00 | + 1 → 34.00 | 27.1 | 5.443 |