Fig. 6

SLINC_8097 suppresses lincomycin biosynthesis. a Identification of ΔSLINC_8097 by PCR and sequencing. Lane M indicated the DNA molecular weight marker. Lanes 1, 2 and 3 indicated PCR products amplified by primer pair JD8097F/R. Lanes 4, 5 and 6 indicated PCR products amplified by primer pair CR1/CR2. 1 and 4, WT; 2 and 5, ΔSLINC_8097; 3 and 6, pKCcas9d8097. b Effect of SLINC_8097 on lincomycin production. c Transcriptional analysis of lincomycin biosynthetic genes in WT and ΔSLINC_8097. The relative expression was normalized using internal reference gene hrdB. The transcriptional level of each gene in WT was set to 1.0. *P < 0.05; **P < 0.01; ***P < 0.001