Fig. 4

SLINC_0469 suppresses lincomycin biosynthesis. a Identification of ΔSLINC_0469 by PCR and sequencing. Lane M indicated the DNA molecular weight marker. Lanes 1, 2 and 3 indicated PCR products amplified by primer pair JD0469F/R. Lanes 4, 5 and 6 indicated PCR products amplified by primer pair CR1/CR2. 1 and 4, WT; 2 and 5, ΔSLINC_0469; 3 and 6, pKCcas9d0469. b Effect of SLINC_0469 on lincomycin production. c Transcriptional analysis of lincomycin biosynthetic genes in WT and ΔSLINC_0469. The relative expression was normalized using internal reference gene hrdB. The transcriptional level of each gene in WT was set to 1.0. **P < 0.01; ***P < 0.001