Table 2 Settings of the GSMM iOpol23 to simulate five different pathway scenarios. The setting of the ‘Unconstraint Reference’ is also the basis for the remaining four scenarios
From: Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha
Scenario | Model Adaptation |
|---|---|
Unconstraint Reference (Ref) | MeOH rate: 10 mmol/gDW/h Objective: malate/acetone/isoprene |
Blocked TCA (TCA-) | = Ref and: Upper bound = 0 for: (PDHa1, PDHcm, ACLSm, AKGDH1 + 2, CITtam, CITtap, CSp + m) |
Forced TCA (TCA+) | = Ref and: Lower bound = 7.8 for: AKGDam |
Forced Glyoxylate shunt (Glx + Std) | = Ref and: Lower bound = 10 for: ICL |
Forced, Impaired Glyoxylate shunt (Glx + Mut) | = Glx + Std and: Lower/Upper bound = -10/2.85 for: MDHm |