Fig. 6
From: Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha
Malate production of O. polymorpha PMT-strain in methanol fed-batch experiment in shake flasks. Malate production (A, D, green squares), optical density (A, D, blue circles), methanol concentration (B, E, red triangles), and pH (C, F, grey diamonds) in shake flasks that were fed with 8 g/L (1%) of methanol every 24 h. Comparison of Verduyn medium with 100 mM of KH-phthalate (A, B, C) and 200 mM KH-phthalate (D, E, F) as buffer. Panels(G, H & I) represent an experiment where shake flasks were fed with a constant methanol feed using the LIS system. (G: OD (blue circles), Malate concentration (green squares), H: Methanol concentration, I: pH)