Fig. 3
From: Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha
Production of malate (A) from methanol and development of optical density (B) in O. polymorpha cultures. Comparison of the unmodified wildtype (WT) strain, a strain overexpressing the PYC and MDH gene of Rhizopus oryzae (PM), a strain overexpressing the malate transporter gene MAE1 of Schizosaccharomyces pombe (T) and a strain overexpressing all three of these heterologous genes (PMT). Cultivations were performed on Verduyn medium in 250 mL shake flasks. Error bars represent the standard deviation of biological triplicates. (C) Illustration of the malate-aspartate shuttle, Used abbreviations: Glutamate (Glu), α-ketoglutarate (α-KG)