Fig. 2
From: Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha
(A) Production envelope of the yield for malate, acetone, and isoprene in response to growth rate. A higher slope of malate indicates stronger consumption with growth. (B) Principal component analysis of simulated flux distributions of five different metabolic scenarios while optimizing for target production: unconstraint reference flux (Ref: black), inhibition of TCA cycle (TCA-: red), enforcing high TCA cycle (TCA+: blue), enforcing glyoxylate shunt (GlxStd: green) and glyoxylate shunt without malate dehydrogenase (GlxVar: magenta). While the absolute position in the plot is irrelevant, the relative distance of points reflects the degree of similarity between flux distributions. Details about the reactions constrained for each scenario can be found in the SI: Figure S1